Table 1 Antibodies table
From: Regulatory T cells limit age-associated retinal inflammation and neurodegeneration
Primary antibody | Cat. No | Company | Host | Dilution |
|---|---|---|---|---|
Brn-3a Antibody (C-20) | sc-31984 | Santa Cruz Biotechnology | Goat | 1:200 |
Ionized calcium binding adaptor molecule 1 (IBA-1) antibody | ab5076 | Abcam | Goat | 1:200 |
Anti-CD68 antibody [FA-11] | ab53444 | Abcam | Rat | 1:200 |
Recombinant Anti-PKC alpha antibody [133] | ab11723 | Abcam | Mouse | 1:500 |
Anti-Cone Arrestin Antibody | AB15282 | Sigma | Rabbit | 1:500 |
Guinea Pig Anti-Synaptophysin | AGP-144 | Alomone | Guinea Pig | 1:150 |
Anti-Glial Fibrillary Acidic Protein antibody (GFAP) | Z0334 | Agilent | Rabbit | 1:500 |
Anti-Mouse MHC Class II (I-A/I-E) Functional Grade | 36–5321-85 | Thermofisher | Biotin | 1:200 |
Anti-Secretagogin | RD184120100 | Biovendor | Sheep | 1:400 |
DAPI (4′,6-diamidino-2-phenylindole) | D3571 | Thermofisher | 1:1000 | |
Secondary antibody | Company | Cat. No | ||
Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 | Thermofisher | A10042 | ||
Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 | Thermofisher | A-31573 | ||
Donkey anti-Rat IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 | Thermofisher | A21208 | ||
Donkey anti-Rat IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 | Thermofisher | a11226 | ||
Donkey anti-Rat IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 | Thermofisher | A78947 | ||
Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 | Thermofisher | A21202 | ||
Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 | Thermofisher | A10037 | ||
Donkey anti-Sheep IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 | Thermofisher | A-11015 | ||
Donkey anti-Goat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 | Thermofisher | A11057 | ||
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129) | Abcam | ab150129 | ||
Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (ab150131) | Abcam | ab150131 | ||
Goat anti-Guinea Pig Alexa Fluor® 568 | Abcam | ab175714 | ||
Alexa Fluor™ 488 Phalloidin | Thermofisher | A12379 | ||