Fig. 4
From: Regulatory T cells limit age-associated retinal inflammation and neurodegeneration
Identification of the biological processes enriched in aged Treg and effect of the secretome in retinal explants. A-B Differentially expressed genes (DEG) in aged Treg when compared to young Treg in Guo et al., 2020 (GSE130419) (A) and de la Fuente et al. 2023 (GSE218804) (B). C, D Venn diagram the genes that are commonly differentially expressed between both datasets. (C) Venn diagram showing the genes commonly upregulated in aged Treg across both datasets and (D) Venn diagram showing the genes downregulated in aged Treg in both datasets. E Quantification showing the fold enrichment of the GO Biological processes enriched amongst the genes upregulated in aged Treg and that are associated with inflammatory processes or nervous system processes. F Quantification showing the GO Biological processes fold enrichment of the genes downregulated in aged Treg. G, H Determination of the effect of aged Treg secretome in retina explants. (G) Representative images of cone-arrestin+ cones (CA+, red) in retinal explants 6h after treatment with LPS, LPS and Treg secretome, control Treg media and Treg secretome in vitro (Scale bar = 50µm) and (H) quantification of the cone survival shown as percentage of the control condition. Data information: E-F n = 4 mice, data presented as mean ± s.e.m. *P < 0.05; 1-way ANOVA followed by Bonferroni's multiple comparisons test